The Affinity of Elongated Membrane-Tethered Ligands Determines Potency of T Cell Receptor Triggering
To produce soluble scFv, the transmembrane anchor sequence in p2C11-B7 was replaced with a polyhistidine tag (6xHis). BC3 and OKT3 scFv sequences were digested by SfiI and SalI and replaced 2C11 in ...
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